Cardinal parameter growth and growth boundary model for non-proteolytic Clostridium botulinum – Effect of eight environmental factors

A new cardinal parameter growth and boundary model for non-proteolytic C. botulinum was developed validated fresh lightly preserved seafood poultry products. 523 rates in broth were used to determine values terms temperature, pH, NaCl/water activity, acetic, benzoic, citric, lactic sorbic acids. The included the inhibiting interactive effect between these factors it calibrated using curves from 10 challenge tests with unprocessed seafood. For evaluation, 40 well characterized performed. Comparison of observed (?max-values) predicted by resulted a bias factor (Bf) 1.12 an accuracy (Af) 1.40. Furthermore, evaluated 94 432 time toxin formation data extracted scientific literature seafood, poultry, meat, pasta prepared meals. These responses 36 different toxigenic strains botulinum. obtained Bf-/Af-values 0.97/2.04 ?max-values 0.96/1.80 formation. correctly 93.8% 5.6% being fail-safe <1% fail-dangerous. cocktail four non-toxin producing Clostridium spp. isolates develop had more than 99.8% 16S rRNA gene similarity (Group II). high number environmental makes flexible tool facilitate development or reformulation products that do not support Further, evaluation is desirable particularly vegetables, meals as dairy present study.